Skip to content

Blast - Basic Local Alignment Search Tool

There is BLAST (called legacy BLAST) and a newer BLAST+ from NCBI (National Center for Biotechnology Information) and it can be confusing. The program names and functions in blast and blast+ are quite different.
See NCBI Homepage: https://blast.ncbi.nlm.nih.gov/Blast.cgi and https://www.ncbi.nlm.nih.gov/books/NBK52640/

blast

Also called legacy blast, it is based on the NCBI C-toolkit.
These old versions can be found here: https://ftp.ncbi.nlm.nih.gov/blast/executables/legacy.NOTSUPPORTED/
Filenames are of the form: blast-#.#.#-CHIP-OS.tar.gz

Some of these versions have been installed under /shared/opt/ with directory names with legacy at the end like ncbi-blast-2.2.26_legacy.

blast+

Newer one based on the NCBI C++ toolkit.
All versions are found here: https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/
The latest version is found here: https://ftp.ncbi.nlm.nih.gov/blast/executables/LATEST/
Filenames are of the form: ncbi-blast-#.#.#+-CHIP-OS.tar.gz
Versions from 2.2.18 onwards.

Some of these versions have been installed under /shared/opt/ with directory names with a plus at the end like ncbi-blast-2.2.22+.